wnt1 polyclonal antibody  (Proteintech)

Journal: STAR Protocols

Article Title: Protocol for evaluating the effects of large gradient high magnetic fields on osteocyte function

doi: 10.1016/j.xpro.2024.103186

Figure Lengend Snippet: Protein expression levels of Wnt signaling pathway (Wnt1, LRP6, β-catenin, and SOST) Data shown as mean ± SD. n = 3. ∗ p < 0.05, ∗∗ p < 0.01; vs. control. ## p < 0.01, ### p < 0.001; 12 T upward vs. 12 T downward.

Article Snippet: WNT1 polyclonal antibody (1:1,000) , Proteintech , RRID: AB_2881013 ; Cat# 27935-1-AP.

Techniques: Expressing, Control

Journal: STAR Protocols

Article Title: Protocol for evaluating the effects of large gradient high magnetic fields on osteocyte function

doi: 10.1016/j.xpro.2024.103186

Figure Lengend Snippet:

Article Snippet: WNT1 polyclonal antibody (1:1,000) , Proteintech , RRID: AB_2881013 ; Cat# 27935-1-AP.

Techniques: Recombinant, Software, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Cell Culture, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Thymopentin-Mediated Inhibition of Cancer Stem Cell Stemness Enhances the Cytotoxic Effect of Oxaliplatin on Colon Cancer Cells

doi: 10.3389/fphar.2022.779715

Figure Lengend Snippet: Effect of TP5 on Wnt/β-catenin signaling pathway. (A–F) Typical Western blot analysis (A) and pooled data of protein expression levels of PI3K-P110 β (B) , WNT1 (C) , P-AKT (D) , P-β-catenin (E) and β-catenin (F) . Histograms are expressed as induction rates in triplicate for the GAPDH control. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01 and *** p < 0.001, one-way ANOVA with Dunnett’s post hoc test, (B) , F (4, 10) = 7.486, p = 0.0047, (C) , F (4, 10) = 11.47, p = 0.0009, (D) , F (4, 10) = 6.387, p = 0.0081, (E) , F (4, 10) = 7.421, p = 0.0048, (F) , F (4, 10) = 1.342, p = 0.3204; ns, not significant.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibody, and secondary antibodies anti-rabbit IgG HRP-conjugated (1: 3,000, 7074S, Cell Signaling Technology) and anti-mouse IgG HRP-conjugated (1: 3,000, 7076S, Cell Signaling Technology) were incubated at 25°C for incubate for 2 h. The primary antibodies used are Beta Catenin Rabbit Polyclonal Antibody (1: 1,000, 51067-2-AP, Proteintech), WNT1 Rabbit Polyclonal Antibody (1: 1,000, 27935-1-AP, Proteintech), Phospho-beta Catenin (Ser33/37/Thr41) antibody (1: 1,000, 9561, Cell Signaling Technology), AKT1 (Phospho-Ser473 + Tyr474) antibody (1: 1,000, 12669, Abcam), PI3K p110 (beta) polyclonal antibody (1: 1,000, 20584-1-AP, Proteintech), AKT1 polyclonal antibody (1: 1,000, 10176-2-AP, Proteintech), and GAPDH (1: 10,000, 60004-I-Ig, Proteintech).

Techniques: Western Blot, Expressing, Control

Journal: Frontiers in Oncology

Article Title: Circular RNA Protein Tyrosine Kinase 2 Promotes Cell Proliferation, Migration and Suppresses Apoptosis via Activating MicroRNA-638 Mediated MEK/ERK, WNT/β-Catenin Signaling Pathways in Multiple Myeloma

doi: 10.3389/fonc.2021.648189

Figure Lengend Snippet: Antibodies.

Article Snippet: Rabbit polyclonal to WNT1 , Abcam (UK) , 1:2000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Circular RNA Protein Tyrosine Kinase 2 Promotes Cell Proliferation, Migration and Suppresses Apoptosis via Activating MicroRNA-638 Mediated MEK/ERK, WNT/β-Catenin Signaling Pathways in Multiple Myeloma

doi: 10.3389/fonc.2021.648189

Figure Lengend Snippet: Circ-PTK2 affected Wnt/β-catenin and MEK/ERK signaling pathways via regulating miR-638. Comparison of pMEK1/2, pERK1/2, WNT1 and β-catenin protein expressions detected by western blot among different groups of U266 cells (A–D) . Comparison of pMEK1/2, pERK1/2, WNT1 and β-catenin protein expressions detected by western blot among different groups of LP-1 cells (E–H) . NS, non-significant; OE-circ-NC, circRNA control overexpression; OE-circ-PTK2, circ-PTK2 overexpression; KD-circ-NC, circRNA control knock-down; KD-circ-PTK2, circ-PTK2 knock-down; OE-miR-NC, miRNA control overexpression; OE-miR-638, miR-638 overexpression; KD-miR-NC, miRNA control knock-down; KD-miR-638, miR-638 knock-down plasmid; * P < 0.05; ** P < 0.01; *** P < 0.001. Experiments were performed in triplicates. The comparison was determined by One-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Rabbit polyclonal to WNT1 , Abcam (UK) , 1:2000.

Techniques: Protein-Protein interactions, Comparison, Western Blot, Control, Over Expression, Knockdown, Plasmid Preparation